منابع مشابه
Lasers for flow cytometry.
Lasers are the principle light sources of flow cytometers. Most modern benchtop instruments feature air-cooled argon-ion laser emitting 10-25 mW of light at 488 nm. This unit covers the various types of lasers available and the qualities that make them suitable or unsuitable for use in flow cytometers. Also included is a discussion of future directions, particularly in the development of solid-...
متن کاملFlow Cytometry
Flow cytometry is a technology that has impacted both basic cell biology and clinical medicine in a very significant manner. The essential principle of flow cytometry is that single particles suspended within a stream of liquid are interrogated individually in a very short time as they pass through a light source focused at a very small region. The optical signals generated are mostly spectral ...
متن کاملFlow Cytometry
Because it has been difficult to identify and separate malignant cells in human lymphoid malignancies, we have developed a flow cytometry-based fluorescent in situ hybridization (FISH) technique using immunoglobulin (Ig) heavy chain variable region (V H) gene probes. After obtaining the specific V H gene sequence expressed by the multiple myeloma IM-9 cell line and the malignant cells in five m...
متن کاملflowFP: Fingerprinting for Flow Cytometry
Abstract Background A new software package called flowFP for the analysis of flow cytometry data is introduced. The package, which is tightly integrated with other Bioconductor software for analysis of flow cytometry, provides tools to transform raw flow cytometry data into a form suitable for direct input into conventional statistical analysis and empirical modeling software tools. The approac...
متن کاملFlow Cytometry: A New Approach for Indirect Assessment of Sperm Protamine Deficiency
Background Flow cytometry (FCM) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. FCM provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. During spermiogenesis histones are replaced by prota...
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ژورنال
عنوان ژورنال: Optik & Photonik
سال: 2010
ISSN: 1863-1460
DOI: 10.1002/opph.201190138